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主营:试剂、抗体、抗鼠、抗人、抗牛、抗猫、抗猪、ELISA试剂盒、流式细胞仪、酶蛋白、ELISpot套件及细胞染色等
℡ 4000-520-616
℡ 4000-520-616
Mabtech/Monkey IgG/IgA FluoroSpot kit/FS-05R24G-2/2 plates
产品编号:FS-05R24G-2
市  场 价:¥0.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:待定
品      牌: Mabtech
公      司:Mabtech
公司分类:
Mabtech/Monkey IgG/IgA FluoroSpot kit/FS-05R24G-2/2 plates
商品介绍
B-cellFluoroSpotkitforenumerationofcellssecretingmonkeyIgG/IgA

TheFluoroSpotassayisaninventivedevelopmentoftheELISpotassayandusesfluorescentdetectioninsteadofcolorimetric.Thisallowsforsimultaneousdetectionofseveralanalytes.ThemonkeyIgG/IgAB-cellFluoroSpotkitenablesdetectionofIgG-andIgA-secretingcellsinthesamewell.

Thekitcontainsmatchedquantitiesofcaptureantibodiesandfluorophore-conjugateddetectionantibodies,lowfluorescentPVDFplatesandafluorescenceenhancer.ThepolyclonalactivatorsR848andrecombinanthumanIL-2areincludedforpre-stimulationofmemoryBcells.

TheantibodiesinthiskitreactwithhumanIgGandIgAandcross-reactwithIgGandIgAfromnon-humanprimates.

Foranalysis,werecommendtheuseofanautomatedFluoroSpotreaderequippedwithfiltersforFITC(excitation490nm/emission510nm)andCy3(excitation550nm/emission570nm).Filtersshouldhavehighspecificitytoavoidbleed-throughartifacts.


AnalyteInformation

IgA
IgA(ImmunoglobulinA)representsonly10-15%ofthetotalIgmoleculesinserumbutisthepredominatingIgisotypeinsaliva,tears,breastmilkandinthegastrointestinalandurogenitaltract.Inserum,theIgAmoleculeiscommonlyfoundasamonomerofeitherIgA1orIgA2subclassbutcanalsoexistasdimersorlargerpolymericforms.TheIgAfoundinsecretions(secretoryIgAorsIgA)iscommonlyadimerwithtwoIgAmoleculesjoinedbytheJchain.SecretoryIgAisthemostimportantIgisotypeinmucoussurfaces,theareaofentryformostpathogens.

IgG
IgG(ImmunoglobulinG)isthemostabundantIgisotypeinserumandmakesupapproximately80%ofallserumImmunoglobulins.Inhumans,therearefoursubclassesofIgGwithhighestserumconcentrationsofIgG1followedbyIgG2,IgG3andIgG4.InmicetheIgGsubclassesaredefinedIgG1,IgG2a/c,IgG2bandIgG3.TheIgGmoleculeconsistsoftwoheavyandtwolightschains(κorλ)resultinginamoleculewithtwoarmsforantigenbinding.HighlevelsofIgGantibodiesareinducedfollowingtheinitialIgMresponseinatypicalimmuneresponsetoantigens.

Application:
  • FluoroSpot
Reactivity:
  • NHP
Contents:
  • CapturemAbs(MT91/145)
  • CapturemAb(MT57)
  • DetectionmAbsanti-humanIgG-550(MT78/145)
  • DetectionpAbanti-humanIgA-490
  • R848
  • RecombinanthumanIL-2
  • Fluorescenceenhancer
  • 2FluoroSpotplates
Size:2plates
品牌介绍

   Mabtech 公司于1986年创办于瑞典斯德哥尔摩大学免疫学系,是一家欧洲的集研究,开发,销售,技术支持为一体的高科技免疫生物技术公司。Mabtech公司始终专业致力于研发新的单克隆抗体,生产创新的免疫试剂和销售高品质的全配套的免疫试剂盒。公司核心业务是致力于优化发展酶联免疫斑点法技术 (ELISpot)、荧光免疫斑点法技术 (FluoroSpot) 和酶联免疫吸附试验技术 (ELISA) 等免疫学方法。Mabtech公司的产品质量体系符合ISO 9001和ISO 13485国际标准。

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