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主营:试剂、抗体、抗鼠、抗人、抗牛、抗猫、抗猪、ELISA试剂盒、流式细胞仪、酶蛋白、ELISpot套件及细胞染色等
℡ 4000-520-616
℡ 4000-520-616
Mabtech/Human IL-6/IL-1beta/GM-CSF/TNF-alpha FluoroSpot PLUS kit (FSP-13121509-2)/FSP-13121509-2
产品编号:FSP-13121509-2
市  场 价:¥24400.00
场      地:美国(厂家直采)
产品分类: 蛋白类>聚合物>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$1220.00
品      牌: Mabtech
公      司:Mabtech
公司分类:
Mabtech/Human IL-6/IL-1beta/GM-CSF/TNF-alpha FluoroSpot PLUS kit (FSP-13121509-2)/FSP-13121509-2
商品介绍
FluoroSpot kit with pre-coated plates for enumeration of cells secreting IL-6, IL-1β, GM-CSF and TNF-α

The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The human /IL-6/IL-1β/GM-CSF/TNF-α FluoroSpot kit is perfect for users who wish to have a convenient assay with minimized assay variability. It enables enumeration of cells secreting one, two, three or four analytes. The kit includes low fluorescent plates pre-coated with monoclonal capture antibodies, monoclonal detection antibodies, secondary reagents conjugated to fluorophores and a fluorescence enhancer. LPS is included as positive control stimulus.

Find out more about 4-color FluoroSpot.

Pre-coated plates enable a more rapid assay with minimized variability.

For analysis, we recommend the use of an automated FluoroSpot reader equipped with filters for excitation 380 nm/emission 430 nm (LED380), excitation 490 nm/emission 510 nm (LED490, FITC) for 490-reagent detection, excitation 550 nm/emission 570 nm (LED550, Cy3) for 550-reagent detection and excitation 640 nm/ emission 660 nm (LED640, Cy5) for 640-reagent detection. Filters should have high specificity to avoid bleed-through artifacts.


Analyte Information

GM-CSFGranulocyte macrophage-colony stimulating factor (GM-CSF) can be produced by several cell types among others activated T cells, macrophages, endothelial cells and stromal cells. GM-CSF stimulates the survival and functional activities of neutrophils, eosinophils, and monocyte-macrophages. It also stimulates the differentiation and proliferation of hematological progenitors.

IL-1βInterleukin-1ß (IL-1ß or IL-1F2, also known as Interleukin-1beta, IL-1 beta, IL1b and Interleukin-1 beta) is a proinflammatory cytokine and inducer of acute phase responses. IL-1ß is produced primarily by monocytes, macrophages, and dendritic cells after induction by microbes.

IL-6Interleukin 6 (IL-6, IL6) is a pleiotropic cytokine that is produced by many different cell types and plays a role in a wide range of functions, such as immune responses, acute-phase reactions, and hematopoiesis. Among other things it augments antibody production from activated B cells in vitro.

TNF-αTumor necrosis factor-α (TNF-α, also known as TNF-alpha, TNF-a, TNFa, and Tumor necrosis factor-alpha) is produced by many different cell types e.g. monocytes, macrophages, T cells and B cells. Among the many effects of TNF-α, are protection against bacterial infection, cell growth modulation, immune system regulation and involvement in septic shock.

Ordering Information

Orders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.

Email:Send your order to order@mabtech.com.

Customers from USA or Canada: Please send your order to mabtech.usa@mabtech.com.
Application:
  • FluoroSpot
Reactivity:
  • Human
Format:FluoroSpotPLUS
Documentation:Datasheet/Protocol SDS
Contents:
  • DIG-conjugated detection mAb (39C3)
  • BAM-conjugated detection mAb (7P10)
  • Biotinylated detection mAb (23B6)
  • WASP-conjugated detection mAb (MT20D9)
  • Anti-DIG-380
  • Anti-BAM-490
  • SA-550
  • Anti-WASP-640
  • Positive control (LPS)
  • Fluorescence enhancer
  • 2 Pre-coated FluoroSpot plates (mAbs 13A5, MT175, 21C11, and MT25C5)
Size:2 plates
品牌介绍

   Mabtech 公司于1986年创办于瑞典斯德哥尔摩大学免疫学系,是一家欧洲的集研究,开发,销售,技术支持为一体的高科技免疫生物技术公司。Mabtech公司始终专业致力于研发新的单克隆抗体,生产创新的免疫试剂和销售高品质的全配套的免疫试剂盒。公司核心业务是致力于优化发展酶联免疫斑点法技术 (ELISpot)、荧光免疫斑点法技术 (FluoroSpot) 和酶联免疫吸附试验技术 (ELISA) 等免疫学方法。Mabtech公司的产品质量体系符合ISO 9001和ISO 13485国际标准。

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